O-090 Endocrine disruptors

Abstract Female mammals, including women, are exposed to endocrine disrupting chemicals (EDCs) on a daily basis. This is of concern because many EDCs inhibit ovarian follicle growth and steroidogenesis, processes that critical for maintaining female fertility. Our research focuses the effects known as phthalates Phthalates interest they ubiquitous synthetic used plasticizers stabilizers in myriad consumer products, everything from shower curtains children’s toys cosmetics personal care products such perfumes, nail polish, deodorants, lotions. also pesticides, wood finishes, adhesives, solvents, lubricants, defoaming agents, medical devices tubing, blood bags, surgical gloves, dialysis equipment. Despite widespread use human exposure, limited information available about environmentally relevant phthalate mixtures reproduction. Thus, we tested hypothesis prenatal exposure an mixture affects reproduction F1, F2, F3 generations mice. To test this hypothesis, pregnant CD-1 mice were orally dosed with vehicle control (corn oil) or (20µg/kg/day-500mg/kg/day) gestational day 10 birth. The was based urinary metabolite levels measured women United States, consisted 35% diethyl phthalate, 21% di(2-ethylhexyl) 15% dibutyl di-isononyl 8% diisobutyl 5% benzylbutyl phthalate. Adult F1 females born these dams create F2 generation by mating them unexposed males males. At selected times, estrous cyclicity fertility indices monitored ovaries sera collected analysis. data indicate significantly decreases anogenital distance, testosterone levels, litter size, but it increases abnormal cyclicity, uterine weight, number cystic ovaries, follicle-stimulating hormone luteinizing compared generation. Further, our body time pregnancy In addition, distance live pups Collectively, suggest adversely reproductive function multi- transgenerational manner These findings have increased understanding mechanisms which cause toxicity. eventually may lead development novel targets prevention treatment infertility induced EDCs.